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KMID : 0923620070070040186
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Immune Network
2007 Volume.7 No. 4 p.186 ~ p.196
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Enhancement of Antigen-specific Antibody and CD8+ T Cell Responses by Codelivery of IL-12-encapsulated Microspheres in Protein and Peptide Vaccination
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Park Su-Hyung
Chang Jun
Yang Se-Hwan
Kim Hye-Ju
Kwak Hyun-Hee
Kim Byong-Moon
Lee Sung-Hee
Sung Young-Chul
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Abstract
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Background: Although IL-12 has been widely accepted to play a central role in the control of pathogen infection, the use of recombinant IL-12 (rIL-12) as a vaccine adjuvant has been known to be ineffective because of its rapid clearance in the body.
Methods: To investigate the effect of sustained release of IL-12 in vivo in the peptide and protein vaccination models, rIL-12 was encapsulated into poly (DL-lactic-co-glycolic acid) (PLGA).
Results: We found that codelivery of IL-12-encapsulated microspheres (IL-12EM) could dramatically increase not only antibody responses, but also antigen-specific CD4+ and CD8+ T cell responses. Enhanced immune responses were shown to be correlated with protective immunity against influenza and respiratory syncytial virus (RSV) virus challenge. Interestingly, the enhancement of CD8+ T cell response was not detectable when CD4+ T cell knockout mice were subjected to vaccination, indicating that the enhancement of the CD8+ T cell response by IL-12EM is dependent on CD4+ T cell "help".
Conclusion: Thus, IL-12EM could be applied as an adjuvant of protein and peptide vaccines to enhance protective immunity against virus infection
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KEYWORD
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Interleukin 12, PLGA microspheres, vaccine adjuvant, CTL response
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